Classical Swine Fever PCR Negative Cohort Study
Partnering to expand testing capacities, support further evaluation and validation of two commercially available CSF PCR assays, and enhance preparedness across the NAHLN.
Research is at the heart of the National Pork Board’s mission and is funded by your Pork Checkoff dollars. Research is administered in all areas of pork production, processing, and human nutrition to develop a higher quality and more profitable product in the competitive meat protein market.
Learn more about how you can help advance the pork industry through ongoing research.
Partnering to expand testing capacities, support further evaluation and validation of two commercially available CSF PCR assays, and enhance preparedness across the NAHLN.
In this study, we aim to explore the potential of mRNA vaccine technology for the development of a subunit vaccine against ASFV. For this pilot project, we focused on assessing the immunogenicity of mRNA vaccines containing four well-characterized ASFV antigens: p32, p54, C-type lectin, and CD2v. Each of these antigens plays a distinct role in the viral lifecycle.
The objective of this study was to estimate and compare the diagnostic performance of a novel indirect enzyme-linked immunosorbent assay (iELISA) for ASF serum antibodies (iELISA), developed by Innoceleris Ames, IA, USA and produced and commercialized by Tetracore (Rockville, MD, USA), and the VetAlert™ ASFV DNA Test Kit (qPCR, Tetracore) in both serum and oral fluid (OF) samples collected on farms in Vietnam.
The first study using experimentally ASFV inoculated adult, commercial boars to determine which sample types and PCR procedures were optimum in detecting ASFV in boars and gilts. Additionally, it proved that insemination of ASFV infected semen to naïve gilts resulted in transmission of the virus.
This research project assessed the African swine fever virus’s (ASFV) nucleic acid detection in slaughter expedient samples: diaphragm meat juice, diaphragm muscle swab, spleen, and spleen swab.