A PRRSV ORF5 database, accessible worldwide, was improved for swine producers, practitioners and researchers (http://prrsv.ahc.umn.edu/). This database, established to fulfill one objective of the PRRS Initiative of the National Pork Board, consists of PRRSV ORF5 nucleotide sequence data generated by diagnostic laboratories on field samples. The sequences assembled and placed into a searchable webpage, such that any user can go from an isolate sequence with its associated RFLP analysis, percent relatedness to modified live vaccines, and predicted N-glycosylation, to generating alignments and phylogenetic dendograms that show nucleotide or amino acid relatedness with minimal clickable steps. It now contains over 5400 sequences with improved alignment viewing, improved sequence comparison viewing, and improved phylogenetic analysis tools. It is the database of choice for selection of vaccine candidates, understanding PRRSV variation, epidemiological queries and other requests. In the future, as the database is expanded to include information from other laboratories, we envision this database as the only website in the world to obtain timely information that is critical to PRRS elimination.