The objective of this research was to develop molecular diagnostic methods for differentiating live, attenuated Salmonella Choleraesuis vaccines from virulent Salmonella-like bacteria.Samples submitted to the Minnesota Veterinary Diagnostic Lab (MVDL) as part of routine diagnostic workup were used for test development. 115 suspected vaccine isolates and 101 known Salmonella Choleraesuis isolates were tested using the newly developed test. 22 additional Salmonella serotypes and 14 unrelated pathogens were also tested to demonstrate the specificity. The multiplex test for the Argus live vaccine strain of Salmonella Choleraesuis was successful in quickly and specifically identifying the Argus vaccine and in differentiating it from virulent strains of Salmonella Choleraesuis. This test is currently being offered for routine diagnostics at the Minnesota Veterinary Diagnostic Laboratory.
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